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The labelled cDNA was then purified using a Qiagen PCR column, pooled with the appropriate sample for competitive hybridization and concentrated to 50 μl on a YM 30 filter.
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Gene expression values for the samples measured for competitive fitness and competitive fitness values for the samples measured for gene expression were treated as missing data (see Appendix).
In an oligonucleotide-tagged multiplex (OTM) assay, a mixture of SH2 domains with domain-specific DNA tags are incubated with a sample spot allowing for competitive binding (single sample per well) [ 8].
Labeled products were precipitated, purified and combined (sample + control) for competitive hybridization on the array slide at 42°C for 72 hours.
We recruited current and never smoker volunteers from Boston Medical Center for buccal (n = 11) and nasal (n = 15) microarray studies, and subsequent buccal epithelial samples for real competitive PCR validation using mass spectrometry (n = 14).
This model of statistical experiment is valid for competitive methods with randomization of samples, such as: GSEA, SAFE.
On Friday, CBS News, in partnership with The New York Times and the online pollsters YouGov, asked voters in all 435 U.S. House Districts about which candidate they are planning to support for U.S. House, with larger samples for the most competitive districts.
Sample posts on a sister forum called StackAdvice include "Good effective nootropic stack for competitive college performance?" and "Seeking stack advice for mood enhancement". Users compare and critique stacks like developers arguing about server software.
For these products, various problems, such as minimal sample size, competitive failure, complex stress and component relevance, often arise simultaneously; and are followed by technical problems.
To test the first- and second-order factorial structure of the Coping Inventory for Competitive Sport (CICS; Psychol. Sport Exercise, 3, (2002) 1 34) with a sample of marathoners.
Except, presumably, for competitive lumberjacking.
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