For single plasmid expression 2 μg of plasmid DNA was used per sample; for co-expression 2 μg of each plasmid DNA was used (unless stated otherwise).
To test this, we immunopurified Flag-tagged Tec1-Ubc9, and Tec1K54R-Ubc9 fusion proteins and probed the purified samples for co-purified Ste12.
We also analyzed 27 astrocytoma tumor samples for co-expression of PTCH1 and GLI1.
The Nyquist plots for a fresh sample of Co-BDCN-24h, measured after 1 and 50 cycles, are shown in Fig. 7.
Results showed that the performance of the two catalysts were similar at low flow rate and with a single reacting gas, while the zoned sample worked better for CO and C3H6 oxidation as the flow rate increased, and better for NO oxidation in the NO/C3H6 gas mixture.
For example the fingerprint ' F CoTr' indicates that a faecal sample was positive for Co and Tr, and negative for all other species tested (Pa, Cl, Sa, Gi, Cr and To).
As a result, a total of 1,081 Affymetrix samples remained for co-expression analysis.
Of 19 samples checked for co-receptor usage, 14 (73.7%) were chemokine co-receptor 5 (CCR5) variants while three (15.8%) were CXCR4 variants.
Because (A_mathrm {ec}) could not be measured for the Co samples, no data for Co are included in Fig. 6b.
Rumen and duodenal samples were analyzed for Co and HMB, and serum was analyzed for free methionine.
The second series was performed 20/120 days after the end of irradiation with measurement times of 24-100 h for sample for determining Ce, Co, Cr, Cs, Eu, Fe, Hf, Hg, Nd, Ni, Rb, Sb, Sc, Se, Ta, Th, Yb, Zn [7, 25, 40].
