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A blood sample for biochemical measurements and DNA extraction was taken from each individual.
All participating subjects were fasting for at least 12 hours prior to collection of blood sample for biochemical and S100B analysis.
All of the patients had undergone assessment of renal function via Cr-EDTA clearance; a blood sample for biochemical analysis had also been taken at the same time.
Each individual provided a blood sample for biochemical analysis and detection of platelet microaggregation under fasting conditions and underwent standard physical examinations; brachial-ankle pulse-wave velocity (baPWV) was also measured.
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Sampling for biochemical markers was standardised by obtaining blood at a fixed hour after an over-night fast.
60 days after end of treatment, 24-hour urine was collected from rats, and subsequently animals were euthanized under anesthesia (zolazepam + tiletamine 1 1, 20 mg/kg) via instantaneous decapitation with blood sampling for biochemical and immunological tests.
Blood samples for biochemical analyses were obtained from the hearts.
At 21 day, mice were euthanized and tissues were sampled for biochemical and histological analysis.
Blood samples for biochemical analyses were drawn in the morning after an overnight fast.
After thawing the saliva samples for biochemical analysis, the fraction of free cortisol in saliva was determined using a time-resolved immunoassay with fluorometric detection.
On day 7, overnight-fasted rats were anaesthetized and then euthanized by CO2 inhalation, 3 h after the last treatment with SSR240612, to collect tissues and blood samples for biochemical and molecular studies.
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