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Compared with dnaPipeTE, RE requires only one sample for assembly and annotation.
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To remove CCN transcripts from raw transcriptomes, RNA-Seq data was mapped to CCN transcripts using SOAP [ 78], and unmapped reads from each sample were used for assembly in the next step.
We used random sampling for the assembly of Ion PGM and MiSeq data and selected the best subset.
Although we used all 6 samples for the assembly of the transcripts, all genes identified had read coverage on each sample (data not shown), suggesting that our RNA sequencing of each sample was deep enough to allow expression analyses for all the genes.
Reads from all samples were pooled for assembly.
The subset sample of 4,243,902 reads (0.3×) was used to assemble TEs and repeats for A. albopictus, consisting of 2 samples of 0.1× genomic coverage for assembly and a third sample of 0.1× for the quantification step.
Samples from assembly reactions were centrifuged at 100 000×g for 75 minutes at 4°C to separate free and aggregated tau.
Samples from assembly reactions were centrifuged at 100 000 × g for 75 min at 4°C to separate free and aggregated tau.
The development of this Fe S signal was absent for assembly complex samples that contained the C35A, C61A, and C104A variants.
As multiple samples from the same species were sequenced, unigenes from each sample's assembly were taken for further processing with the sequence clustering software TGICL, for sequence splicing and overlap removal to acquire non-redundant unigenes that were as long as possible [ 33].
Eight additional individuals (three territorial males, three females and two sneaker males) were collected and used as a pooled sample for the de novo transcriptome assembly.
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