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An enrichment factor of 21.4, a detection limit of 2.7 ng l−1, along with a sampling frequency of 13 h−1 were obtained at a sample flow rate of 6.0 ml min−1.
Since very low sample flow rate is mandatory for single-virus sorting to prevent doublets, virus sample flow rate was adjusted to 40 50 events per second.
Sample flow rate was confirmed to be among the most significant factors to affect sensitivity.
The selected factors were: sorbent mass, sample pH, buffer concentration and sample flow rate.
Adsorbent mass and sample flow rate were evaluated using the Doehlert Matrix.
In the optimization procedure, four variables (sample pH, eluent concentration, sample flow rate and eluent flow rate) were investigated.
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Six experimental variables were optimised: sample pH, sample flow-rate, eluent concentration, eluent volume, eluent flow-rate and minicolumn diameter.
The effects of sample concentration, processing capacity, sample flow-rate and elution flow-rate on purification efficiency were investigated using single and response surface experiments (RS M.
The effect of the sample flow-rate, exposure time to the herbicide, biomass loading, biosensor film thickness, intensity of the actinic light, illumination cycle, and temperature on the biosensor response has been evaluated using waterborne simazine as test bench.
In this study, solid-phase extraction using the imprinted polymer has been optimized with the experimental design approach for a triazine herbicide, named ametryn with regard to the critical factors such as sample pH, sample concentration, sample flow-rate, sample volume, elution solvent, washing solvent and sorbent mass.
The optimal conditions for the purification of G. lucidum crude polysaccharides with RFC were determined: sample concentration of 10 mg/ml, sample volume of 100 ml, sample flow-rate of 2 ml/min and elution flow-rate of 40 ml/min.
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