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The same sample, filtered through a 0.5 μm pore-size membrane, yielded a MW that was at least one order of magnitude lower.
To a sample (filtered) of 25 ml, 1 ml of ammonium molybdate reagent and three drops of stannous chloride reagent were added.
A volume of 500 ml of each collected water sample filtered with Whatman 41 filter paper was taken in a clean weighed porcelain dish followed by addition of 4 gm of cellulose powder and evaporated on water bath.
(a) Size distribution of the as- prepared sample (black line), sample filtered by the 1-μm syringe filter (green line) and sample made from the sediment remained on the filter (red line) obtained by dynamic light scattering (DLS).
Briefly, preserved water samples were vacuum filtered onto 0.2-μm-pore-size blackened polycarbonate filters (Nuclepore) and the cells collected on the filter were stained with AO at a final concentration of 0.01% for 3 min. The volume of sample filtered for cell counts was chosen such that the layer of pyrite particles that accumulated on the filter would not obscure the bacteria.
At approximately 10 and 20 hours from the time the samples were onboard, subsamples were drawn from the refrigerated sample, filtered, and the filtrate was analyzed for NH4+-N concentration.
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Fig. 10 The SEM image and elemental mapping images of the sample filters obtained at the GD site in July 2015.
All samples filtered by using a syringe equipped with a 0.2 micron filter.
Samples filtered followed by equilibration (typically 5 min) in a quartz cuvette to 37°C.
Bulk water chemistry measurements followed standard protocols [90] using samples filtered through washed 0.22 µm Millex GV filters.
In contrast, all samples filtered by the original water filter and all but one of the water samples filtered by the modified filter type remained Legionella-free.
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