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For each sample, expression of marker genes was normalized to cyclophilin.
DGE is very similar in the sampling approach to long oligonucleotide probe microarray hybridization, given that both techniques take short nucleic acid target sequences to sample expression of longer RNA molecules containing them, and both are 3' biased because they rely on extension of cDNAs from the polyA tail with a oligo-dT primer.
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For MMP2, high levels of expression were detected in 20/22 SG samples, while only 2/22 samples expressed moderately; in all GC samples, expression of MMP2 was high (17/17).
Only in three RCC samples expression of HYAL1 decreased more than 10-fold and in one case we detected similar strong decline of HYAL2 expression.
In RCC samples expression of HYAL1 declined in 10 of 15 cases (67%, P<0.02) and average decrease was 6.5 times (from 2 to 46).
On the other hand, in PTC samples, expression of HBB was 15 98% of normal thyroid.
In patient samples, expression of the Hes1 gene inversely correlated with survival [ 41, 48– 50].
In all samples, expression of Tic20 was analysed in direct comparison to Tic110.
While Crlf1 expression is up-regulated in the E-IAA positive samples, expression of the Crlf3 gene is down-regulated.
Indeed, in DCIS- and LCIS-containing samples, expression of decorin mRNA was localized merely to the peritumoral stroma.
For the Bgt test, using inoculated leaf samples, expression of 9,114 DEGs was detected, of which 6,292 genes exceeded 1 kb.
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