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A relative amount for each gene examined was obtained from a standard curve generated by plotting the cycle threshold value against the concentration of a serially diluted RNA sample expressing the gene of interest.
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No sample expressed the HMGA2/NFIB fusion gene.
One sample expressed the marker poorly.
Likewise, for samples expressing the ALT phenotype, three samples expressed the inactive beta variant and only one sample expressed the active wild-type variant.
Five of the 14 colonies we selected as a representative sample expressed the EGFP indicator gene, and were shown by PCR analysis to contain the EGFP gene replacing the gD gene (data not shown).
As was the case with expression levels for hTR, levels of acetylation in those samples expressing the ALT phenotype lie somewhere between those those were either positive for telomerase or negative for both telomerase and ALT.
The relationship between binding of these chromatin modifications and hTERT expression is less obvious, as there are no significant differences between those samples expressing the wild-type variant and those that do not.
In this study, inoculation of Pinus radiata with Phytophthora pluvialis resulted in the reproduction of characteristic needle lesions consistent with those from which the pathogen was most consistently isolated from field samples expressing the disease.
ABP1 was still detected in root samples expressing the scFv12 whereas the protein was not detected in induced antisense samples (Figure 1C).
In CENP-E depleted samples expressing the chimeric Hec1 protein the frequencies of disorganized prometaphases showing multipolar or bipolar spindles dropped dramatically and a vast majority of cells showed many unaligned chromosomes, similarly to CENP-E depleted cells (Figure 7B).
Are different samples expressing the same gene isoforms?
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