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One sample expressed the marker poorly.
No sample expressed the HMGA2/NFIB fusion gene.
Likewise, for samples expressing the ALT phenotype, three samples expressed the inactive beta variant and only one sample expressed the active wild-type variant.
Five of the 14 colonies we selected as a representative sample expressed the EGFP indicator gene, and were shown by PCR analysis to contain the EGFP gene replacing the gD gene (data not shown).
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A relative amount for each gene examined was obtained from a standard curve generated by plotting the cycle threshold value against the concentration of a serially diluted RNA sample expressing the gene of interest.
Even in the case of Annexin-A2, where a sizable proportion of TFL samples expressed the antigen (37%), the level of expression was much lower than that in the corresponding tumour samples.
Gene expression analysis showed that 90% (116 out of 128) of the tumor samples expressed the messenger in the pre-treatment and post-treatment biopsies regardless of methylation status, hence were not informative.
Both approaches demonstrated that stroma samples expressed the highest values of COX-2, closely followed by MSCs and then bladder and prostate cancers, demonstrating that COX-2/PTGS2 is a hallmark of the mesenchymal phenotype (Figure 2h and Table S4).
All tumour and normal samples expressed the VEGF-A isoforms in order of decreasing abundance, 165>121>189.
All examined samples expressed the M1, M2 and M3 receptor subtypes.
Two samples expressed the monocyte/macrophage lineage markers CD163 and CD14, and two others CD68.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com