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Mutant alleles in genomic DNA were quantified to the level of 0.25 ng of mutant DNA in 50 ng of wild-type DNA for a 25-μl sample, equivalent to DNA from 42 mutant cells.
Fatigue and failure tests were conducted on a 30 foot (9.144 m) long by 2 foot (609.6 mm) wide prototype deck sample, equivalent to a quarter portion of the bridge deck.
For normalization of the values of the viral load and the innate immunity gene expression, the mean copy number of β-actin transcripts in each sample, equivalent to 1 μg, was determined from at least ten independent experiments (n ≈ 30); the results of the viral infection and gene expression analysis were indicated as the logarithm of the copy number.
Approximately 30 nl of each sample (equivalent to the amount of material from 40 lysed cells) were arrayed onto nitrocellulose slides (Whatman, MA) by direct contact printing using a high-resolution 2470 arrayer (Aushon Biosystems, Billerica, MA).
The undiluted sample (equivalent to 15 mg of tissue) was loaded onto homemade acrylamide SDS-polyacrylamide gel (PAGE).
A sample equivalent to 10-20 embryos was used to check whether and the shearing of chromatin was successful.
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The August 2008 ash samples have higher SiO2 contents than do most of the 2011 rock samples (equivalent to erupted magma) and past ejecta (Fig. 12).
To remove the IFs of non-seizure components, this threshold is set to the minimum time duration of a seizure component in samples (equivalent to 10 s). .
The non-overlapping sliding window technique was also applied, with the window size defined as 512 samples, equivalent to a 4 s window length.
The supervisor, a Finite State Machine (FSM), then triggers the writing into a buffer that stores 8 k filter output samples (equivalent to the length of an OFDM symbol).
Samples equivalent to 1 µg/band and 15 µg/mL Flutamide were prepared from cytomed-250® tablets solution and were analyzed by HPTLC and HPLC methods, respectively.
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