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Also, an electrokinetically controlled DNA hybridization microfluidic chip has been demonstrated and can perform all processes from sample dispensing to hybridization detection within 5 minutes [ 1].
A natural extension of HT-FCS would consist of combining it with microfluidic sample dispensing technology, in order to observe a different sample in each excitation spot.
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Assay and sample mixes were then automatically loaded into the nanowells of a MyDesign SmartChip with WaferGen's MultiSample NanoDispenser using a '384 assays × 12 samples' dispensing layout.
Serum was isolated from whole-blood samples, dispensed into aliquots and stored at +4°C until processing.
Unfortunately, the database does not contain information on professional samples dispensed at hospital discharge.
Raman spectra of aqueous BSA-SWNT dispersions (500-μL samples dispensed into 35-mm glass-bottom imaging dishes (MatTek)) were acquired using a 50×/0.5 NA LM-Plan objective.
Unlike passive drool techniques that can be embarrassing to the subjects, the saliva samples dispensed by the collection kit were easily collected and devoid of any particulates or air bubbles.
In the monitor, a series of operations for the detection, i.e., sampling and dispensing of the sample, addition of relevant chemicals, acquisition and transmission of the measured data, were fully automated.
After each designed reaction time (30 240 min), the treated sample was dispensed into a glass cylinder and filtered through 0.2 μ filter paper.
Triplicates of each sample were dispensed into 96-well plates and incubated at 37°C.
We centrifuged 4 ml of the sample and dispensed the supernatant into aliquots, which were stored in liquid nitrogen.
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