Unlike other methods of assessing protein turnover which historically have used short term i.v. infusion of radiolabelled tracer or invasive punch sampling (discussed above) in this study subjects drink pre-bottled D2O at home and are then tape stripped.
Following the methodology in Hayem-Ghez et al. [13], hyperspectral pseudo-colour images of red and blue watercolour pigment standards from the samples discussed above were rendered by identifying areas of their Vis/VNIR reflectance spectra showing the most significant differences.
High variance between the cmsHA89 samples (discussed above) likely contributes to the lower number of significantly-differing transcripts detected in comparisons involving this accession.
To search for C. reinhardtii or closely related Chlorophyceae species in the water samples discussed above, we mixed algae in the samples with an mCherry/VHH B11 chimera prior to examination by confocal microscopy.
Furthermore, we were not able to find evidence of this gene fusion expressed in the panel of 57 breast cancer samples discussed above, suggesting that it is not recurrent.
In addition to this comparison between the RMe and RMg samples discussed above, we computed all between-strain-within-condition, and between-condition-within-strain Pearson correlations for the BYg, RMg, RMe, and RMg samples [see Additional file 1, Table S4].
AFM analysis was also performed for the three samples discussed above.
This is, however, distinctly smaller than the true density as calculated for the most complete sampling volume discussed above and is therefore an underestimate.
In addition to the general sample preparation points discussed above, each of the individual parts of the eye require a more empirical approach.
As for the highly sensitive species identification, discussed above, sample processing (e.g., tryptic digestion, acidic/organic extraction, nano-liquid chromatography) can greatly increase the sensitivity and specificity of a MALDI-TOF MS method and thus enable subspecies identification [66, 75].
As we have discussed above, samples of biological origin are often not homogeneous and their MSD does not follow a well-described analytical function.
