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A positive control (a sample known to be positive for the template) and a negative control (a sample devoid of template) were included in each reaction.
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Briefly, homogeneous honey samples devoid of air bubbles were transferred into a cuvette with a 10-mm light path until the cuvette was approximately half full.
In addition, six normal liver samples devoid of any cancer were included in the study.
No samples devoid of imidazol yielded any oligomeric species higher than dimer.
AThTP was present in 35% of the samples containing AThTP, but in only 3% of the samples devoid of ThTP.
Indeed, among the samples containing ThTP, 53% also contained AThTP, while AThTP was only found in 10% of the samples devoid of ThTP (p = 0.0006).
Blank samples, devoid of β-carotene, were prepared for background subtraction.
Negative and positive controls were amplified in every experiment, as well as samples devoid of DNA.
Detection of PrPSc by PMCA in hamster plasma samples devoid of platelets suggests the possibility of cell-free PrPSc in hamster plasma [ 25, 26].
Briefly, homogeneous honey samples devoid of air bubbles were transferred into a cuvette with a 10 mm light path until the cuvette was approximately half full.
After 24 h, EHEC grew by 0.5 log CFU/mL in control samples devoid of any PDA, whereas the bacterial count in samples containing PDAs except EG decreased significantly (P < 0.05).
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