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For the determination of the catalase content, 50 μg protein of each sample (determined by Biorad total protein assay (Bio-Rad LaboraThe Netherlandsenendaal, The Netherlands) was separated on a 12.5% SDS PAGE.
The study of RNA species expressed by a microbial community present within an environmental sample determined by DNA sequencing of reverse transcribed cDNA generated from the RNA.
Sheared chromatin was diluted 1 5 in RIPA Buffer without SDS (with inhibitors) to reduce the sample SDS concentration to 0.1%, and the protein content of each sample determined by Micro BCA Protein Assay Thermo Scientificc).
In the first trial, proportions of damaged sperm cells in each sample determined by the automated procedure agreed closely (r2 = 0.98, P < 0.001) with the predicted theoretical values.
The concentrations of Cl and Fe ions in the effluent sample determined by our sensor are 97.85 ppm and 0.274 ppm respectively.
The mean grain size of the peened sample determined by transmission electron microscope is about 8 ± 2 nm in the top surface layer.
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Figure 2 The surface morphology (2 × 2 μm 2 detail) of PLLA samples determined by AFM.
Fifty-five bottom sediments were collected and the mineralogical composition of six pulverized samples determined by XRD.
The average crystallite size of the samples determined by Debye Scherrer formula ranges from 4.2 to 36.9 nm.
The textural properties of all the samples determined by nitrogen sorption experiments are summarized in Table 1.
The results of all the 269 serum samples determined by the three methods are shown in Table 1.
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