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Results are expressed as a ratio of the optical density of the test sample over that of a reference sample (cut-off for positivity is 1.1).
For the latter markers the morphology and location of positive cells was determined, and a positive sample cut-off was not established.
To identify cases, a cut-off was set at the value of the mean + 1 standard deviation in a nonpsychiatric patient normative sample (cut-off = 0.69) [ 31].
The detection limit was 10 genomic equivalents (GE) per 20-µL reaction (cycle threshold 37.71), corresponding to 4 × 10 GE/g of fecal sample (cut-off cycle threshold 38.00).
The HOMER software command "getDifferentialPeaks" was used to find ChIP-seq differentially enriched regions between different IMEC samples (cut-offs = twofold change and p-value 1×10−4).
The relevance of the sampling cut-offs to the interpretation can be seen with the small sample size (18 20 participants) in the 6-month follow-up group (170 190 days).
Ct-value averaged from at least two duplicate samples, cut-off value 36.0.
Ct-value averaged from two duplicate samples, cut-off value 38.0.
Zip - I used a zip sound from a sample pack, cut off most of the tail from the sample and then pitched down until it was in key with the rest of the track.
Each frozen prostate tissue sample (1 3 mg, 5 13 mm) was weighed and then a piece of the sample was cut off with scissors.
We use a sample adaptive cut off for variation inferred from the data to account for a varying noise level between samples.
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