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Briefly, single larvae were homogenised in a proteinase inhibitor cocktail (Sigma®, St Louis, MO P2714) and a sample corresponding with 10 µg of protein was separated using SDS-polyacrylamide gelectrophoresissis (PAGE).
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The calibration process for the Bai Wierzbicki material model is based on fifteen tested samples corresponding with the literature.
In this sense, it is interesting to note the reversion in the ER immunoexpression status in feline IMC case 2, which was ER-negative when the animal did not have inflammatory signs and ER-positive in the samples corresponding with clinical IMC.
The age distribution of our sample is corresponding with studies from similar populations in Norway.
This is likely the result of the decreasing sample size corresponding with increasing levels of mental health problems.
Titers reported are the reciprocal of the calculated sample dilution corresponding with an A490 ≥ 0.2 after background correction.
In a second experiment, 14 samples corresponding to subjects with HI titters equal or higher than 40 (conventionally considered as exposed to the virus based on the HI assay) were tested by ELISA.
The Test Set MCI is composed by 47 samples corresponding to subjects with MCI as illustrated in Figure 1.
In the samples corresponding to wells with no cells (negative controls), we see only one band corresponding to the unphosphorylated Akt substrate.
A gap of 24000 samples (corresponding to half a second with the selected sample rate) was chosen between the individual excitations.
We searched for raw data of patient-derived, untreated cancer samples with corresponding normal samples deposited in the ArrayExpress (2) or the GEO (3) repository using the HG-U133 Plus 2 array from Affymetrix.
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