Exact(1)
Samples were automatically loaded across a Paradigm Platinum Peptide Nanotrap (Michrom) pre-column (0.15 x 50 mm, 400 µl volume) for sample concentrating and desalting, at a flow-rate of 50 µl/min in HPLC buffer A prior to loading into an inline analytical capillary column (75 µm x 12 cm) with C18 resin (5 µm, 200A° Magic C18AG, Michrom) and Picofrit capillary tubing (New Objective, Cambridge, MA).
Similar(59)
The physicians' use of them also tended to be medium to high, with 70.7 % of the sample concentrated in this segment.
Eggs from each sample, concentrated on a cover slip, were identified to parasite species by scanning five transects under a microscope (100× magnification, Pedersen 2005).
Human volunteers rated 13 different commercial pet food samples, concentrating on 18 so-called flavour attributes: sweet; sour/acid; tuna; herbal; spicy; soy; salty; cereal; caramel; chicken; methionine; vegetable; offaly; meaty; burnt flavour; prawn; rancid; and bitter.
PCR products of the expected size were obtained from samples concentrated up to 102 cfu ml− 1.
Samples concentrated using the Centrifugal Filter Units produced higher levels of CRP, IgE and myoglobin.
Our sampling concentrates on the Central American and northern South American portions of the P. parnellii range and includes individuals from ten countries.
In order to confirm the formation of a mini-cellulosome in vivo, we carried out native PAGE analysis on samples concentrated by ultrafiltration.
We generated a high-density time course of gene expression during 7 days of erythroid (E) and neutrophil (N) differentiation, with sampling concentrated over the first 72 hr.
Aliquots (1.0 ml) of sample concentrate from water and plankton sampleswere added to doubly concentrated alkaline peptone water (APW).
Duplicate dishes were inoculated with 100 µl of a sample (concentrate) after rising aspirate plates with phosphate buffered saline (PBS).
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