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Eggs from each sample, concentrated on a cover slip, were identified to parasite species by scanning five transects under a microscope (100× magnification, Pedersen 2005).
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The number of faecal samples collected varied among the farms, but usually up to 100 samples were collected, with the sampling concentrated on young stock, as they are considered more likely to harbour the bacterium.
Our sampling concentrates on the Central American and northern South American portions of the P. parnellii range and includes individuals from ten countries.
Then, the sample mixture is aspirated through the membrane coated with anti-CRP antibodies, and all CRP in the sample is concentrated on this membrane.
The supernatant was then removed and the sample was concentrated on a C8 HLB Cartridge (Waters Oasis) into 1 mL of MeOH.
To impose some order on our sampling we concentrated on wines from the premium cabernet growing region in the state, the Napa Valley, with a few Sonoma wines added for contrast.
Post extraction, the samples were concentrated on rotavapor and analyzed by HPLC.
The plots of gossypii group samples were concentrated on the mid-left side.
Swabs of environmental surfaces were directly plated; water samples were concentrated on a 0.22 µm pore size filter (Millipore), suspended in saline and plated for P. aeruginosa counting; other liquid samples were appropriately diluted prior to plating.
The protein digests were resolved in 15 µl of 0.02% formic acid in 0.5% acetic acid, and the samples were concentrated on a MGU30-C18 trapping column (LC Packings) and a nano-column (C18 reverse-phase column, Proxeon) at a flow rate of 120 nl/min.
Although all infections detected were among songbirds (Passeriformes), the sampling also concentrated on songbirds (94.3%).
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