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For all iTRAQ sets, a reference sample comprising a pool of the ten normal proliferative samples (100 µg lysate from each tissue) was used.
A heterogeneous sample comprising a mixture of cells and cell media solution was loaded into a 1 ml syringe reservoir and each droplet was printed at pre-determined positions on a glass surface or into a 96-well plate.
The four variants were genotyped in a combined study sample comprising a total of 18,014 subject ascertained from, the population-based Inter99 cohort (n = 6,514), the ADDITION screening cohort (n = 8,662), a population-based study sample (n = 680) and a type 2 diabetic patient group (n = 2,158) from Steno Diabetes Center.
To obtain a mixed selection of the material, we created a sample comprising a total of 20 case reports by using case numbers five and fifteen from each course.
qRT-PCR samples were prepared in triplicate, with each sample comprising a 25-μl reaction mixture in a MicroAmp optical 96-well reaction plate sealed with an optical adhesive cover (Applied Biosystems).
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A convenience sample comprising an anxious group (n = 41) and non-anxious group (n = 33) completed tasks that measured number of self-generated approach goals and avoidance goals, and number of associated positive and negative consequence steps for goals.
Second, we did not train SVMs on the entire training set, but instead on a balanced sample comprising an equal number of relevant and irrelevant examples.
The sample comprised a total of 70 female patients (840 teeth).
The sample comprised a subgroup (n = 50) of participants from the South London Child Development Study.
The sample comprises a colored ground layer composed of chalk and red earth pigment (Fig. 5b, layer 1).
First, our clinical sample comprises a specific population of patients with migraine attending a third-level center and may reflect referral bias.
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