The segmentation results of the real product samples have been evaluated by manual inspection of the dataset, since pyrolizing the sample components was not yet possible.
The sample components were separated in the HP-5 MS (30 m × 0.25 mm × 0.25 μm) capillary column using the following temperature programming: 60°C (3°/min), 240°C/10 min. The injector temperature was 220°C and the interface temperature was 250°C.
The glass sample components are 25TiO2 27BaCO3 8Ba(NO3)2–6ZnO2–9CaCO3–5H3BO3–10SiO2–7water glass-3Er2O3 (wt%), and the emission spectra of TBS glass and a TBS glass microsphere (about 48 μm in diameter) were measured under 633 nm excitation and discussed.
Sample components were separated on a reverse-phase column (Uptisphere C18 5 µm, 150×2 mm i.d, Interchrom) using a Waters 2695 separation module (Alliance) (Waters, Milford, MA, USA) equipped with a Waters 2487 dual UV detector, with a flow-rate of 0.15 ml/min and a binary gradient: acetonitrile 0.5% acetic acid (v/v) (A) and acetic acid 0.5% (v/v) (B).
Some sample components are highly polar whereas others are non-polar.
Ionization of the sample components was performed in electron impact mode (EI, 70 eV).
The separation of CAP from the sample components was carried out using LC or by VHPLC.
The sample components were mixed at ∼0 °C and immediately frozen in copper sample holders (10 mm × 5 mm × 0.5 mm) covered with Mylar using liquid nitrogen.
The sample components were separated by SDS-PAGE, and the TED-fibrinogen Aα adduct bands excised for analysis by MS. For comparison lysine-acetylated fibrinogen bands were produced by the same protocol, with the omission of the TED reaction step.
A new sample component is presented for the Monte Carlo, ray-tracing program, McStas, which is widely used to simulate neutron scattering instruments.
