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Moreover, several of these techniques include reactions that do not have to be completed, and so the sample complexity increases.
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Moreover, side reactions such as glutamine or asparagine deamination and their subsequent methylation had also occurred, so the sample complexity increased (Larsen et al. 2005).
Their elimination from the sample greatly reduces the sample complexity and increases the likelihood of identifying target proteins of the small molecules under study.
Proteome fractionation with anion exchange chromatography was used to reduce sample complexity and increase quantitative coverage of protein expression.
The prefractionation can effectively reduce sample complexity thereby increasing detection sensitivity, which is necessary for identifying low abundance proteins [ 18].
Despite such limitations, SIP is a very valuable tool to reduce sample complexity and increase the hit rates of particular target genes (Chen and Murrell 2010).
It is important that these methods are applied prior to the phosphopeptide enrichment in order to reduce the sample complexity and increase the efficiency of the enrichment.
To decrease sample complexity and to increase the sensitivity of detection we used the CPLL technology.
The percentage of pools in which at least one false positive was identified increased with increasing sample complexity (Fig. 2).
Even though proteomics practitioners are accustomed to bottom-up methodologies; the digestion process results in increased cost, increased run time and grossly increased sample complexity as compared to classical ELISA.
Unsurprisingly, the study concluded that assembly quality decreased rapidly with increasing sample complexity.
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