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A new relatively low-cost sample collection design has been developed using mostly off-the-shelf parts that allows for permanent, internal, three-dimensional sample collection in wetlands.
Sample collection design: TN and YT.
AR carried out stress treatment and sample collection, design and execution of microarray, transcriptome sequencing, QGE analysis, data integration, and drafted the manuscript.
Importantly for researchers employing population genetic analyses for the inference of evolutionary process, these results indicate that variation in sample collection design do not necessarily bias the conclusions drawn on limited data.
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MPRD designed and coordinated the study, contributed to sample collection, questionnaire design and data analysis, and drafted the manuscript.
RH participated in sample collection, study design and manuscript preparation.
MRK and SS made contribution in sample collection and design.
MR: post-mortem sample collection; primer design; RNA extraction; DNase treatment; interpretation of results.
ME, DM and EA made contribution in sample collection and design.
NAS, NAR, and AA participated in sample collection, study design, and proof- reading of the manuscript.
The study sample, data collection, design, consent and confirmation of ethical approval from the East London ethics committee have previously been reported in detail [ 21].
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