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Only information about age, sex, sample collection date and collecting laboratory was available for these samples.
Three replicate 100 ml water samples were collected from each monitoring site on each sample collection date (APHA, 2005) to take into account variability of sampling and testing (repeatability/reproducibility of microbiological analyses).
Plasma was collected from the beginning (6 May) to the end (8 August) of the breeding season, with most samples being collected during the first half of the breeding season (average Julian sample collection date ± SD = 164, or 12 June, ±22; mean Julian clutch initiation date ± SD = 149.7, or 29 May, ±10.7).
Although bacterial species in leachate and bulk samples varied with sample collection date, eight species were identified in samples from multiple sampling dates.
The sample collection date of the strains selected to the phylogenetic and molecular analysis covered the period from May 10 , 2009to February 2 , 2010
Because a certain delay may exist between the sample collection date and the event observation date, the time score is decreased according to the difference between the event observation date and the sample collection date.
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Meteorologic data for the sample collection dates were obtained from the Japan Meteorological Agency.
Differences in sample collection dates, geographic location, and other variables could affect results, although no evidence of any confounding effect was detected.
We used the interval between the last dead bird and the sample collection dates as a potential reflection of the survival of the virus in a natural setting.
However, because the sample collection dates and/or the host species and/or the sample collection location of those isolates might be more or less precise, the computed links of the suggested isolates will have different scores.
The chosen controls were matched to the index case on age at study enrollment (±3 years), dialect group (Hokkien, Cantonese), menopausal status at sample collection, dates of study enrollment (±2 years) and of blood collection (±6 months).
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