Your English writing platform
Discover LudwigSuggestions(1)
Exact(3)
However, this method requires cell solubilization, and the sample cells are killed and, thus, cannot be used for another assay.
This method is superior to ATP and other proliferation assays for drug screening, because sample cells are killed in those assays and cannot be used for subsequent assays.
Trapping manipulations on the apoptotic sample cells are performed in a plain microfluidic chip which is illustrated in Figure 1(b), employing several microliter samples for each measurement.
Similar(57)
Therefore, cleaning the quartz sample cells is very important.
For control culture (0-hour sample) cells were incubated at 37°C in suspension with fresh medium for 30 minutes.
To optimize recovery of bacteria present in the sample, cells were fixed and recovered on a 0.45 µM filter after phage infection as described above.
In a another sample, cells were preincubated overnight in serum-free medium, followed by incubation for 3 h with 500 nM DBT and 10 nM dexamethasone.
The sample cells were collected and counted by trypan blue exclusion using a hemocytometer.
For the 0 hour sample, cells were collected before deprivation of combined nitrogen.
To this end, one million PBMCs or ~250,000 750,000 TDLN or tumor sample cells was used per condition.
From each sample, cells were immediately pelleted by centrifugation (12,000 g, 30 sec, 25°C) and snap-frozen in liquid nitrogen.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com