In such a study, a sample (cell line) is followed over time for a certain period and, at multiple time points during this period, is interrogated molecularly.
The paired sample cell line model from patient 2295, will allow for further discrimination of acquired resistance, affected by the chemotherapy regime.
The samples (cell lines, tumor, PBMC) used for ECL were prepared based on the Meso-Scale Discovery (MSD) lysate preparation protocol Meso-Scale Discoveryy, Gaithersburg, MD) using complete lysis buffer with protease and phosphatase inhibitors.
We have demonstrated that predictive models of EGFR TKI sensitivity can classify both out-of-sample cell lines and lung adenocarcinomas.
In one study, a panel of five miRNAs were studied in 27 FFPE tissue samples, cell lines and samples from a rat model.
Mutated reference samples (cell lines), wild-type DNA (placenta), dewaxing, extraction, and PCR negative controls were included with the samples to be analysed.
Hierarchical clustering of each species' tumor samples, cell lines and normal tissues was performed individually using Euclidian distance and simple linkage [ 50].
Our study aimed to investigate the role of AXL in WDLPS, DDLPS, and PLS through the analysis of patient samples, cell lines, and xenograft models.
The specimens, including skeletal material, tissue samples, cell lines, and chromosomal preparations were deposited at the Natural Science Research Laboratory (NSRL) at the Museum of Texas Tech University under the identification codes TK163823 – TK163823.
HG, SS, RAG, DJ and SN dealt with the provision of study materials, including the procurement of resources and samples (cell lines or reduction mammoplasty and tumor tissues).
