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Next to the composition of the blood sample, calibration of the instrument is equally important and may often add to unknown errors.
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Testosterone metabolites were identified and quantitated by HPLC. 100 μL of sample, calibration standard, or quality material standard were pipetted into separate microcentrifuge tubes and mixed with 100 μL of methanol.
Remaining metabolites, including selected glucuronides, Tam- N-Oxide, 4′-OH-Tam and 4′-OH-NDM-Tam derivatives, as well as selected (E)- and (Z -isomers, have been quantified separately (see Additional file 2 for the examples of sample calibration chromatograms).
This was enriched to over 90% (range 90 96%) in each case with one sorting run per sample after calibration of the machine and settings for each sample.
The same as in the case of pressure measurements before each test of the sample, the calibration of the entire system was performed.
As matrix match is impossible for seized materials due to the unavailability of sample blanks, calibration of an instrument is normally performed with standards directly prepared in the target solvent.
Blood-based methods have uncertainties, including the timing and handling of the blood samples and calibration of the instruments.
The LBD1-CT proteins were used as the reference samples for calibration of the ELISA.
For the MCQ sections, the IRT Rasch model provides sample-independent calibration of item difficulties and estimation of candidate performance [ 5].
To enable accurate pathogen quantification of unknown samples, calibration curves were constructed for each of the 13 PRI-lock probe targets.
As before (Heilmann et al. 2006), the accuracy and reliability of the data were ensured by including quality control serum samples [excess serum samples from the German External Quality Assessment Scheme (G-EQUAS) round-robin program as well as spiked serum pools] in each analytical batch of samples, calibration standards, and reagent and serum blanks.
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