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First, we define the fuzzy decision of a sample by using the concept of fuzzy neighborhood.
Furthermore, we obtain the 2D mapping image of the leaf blade sample by using the ellipsoidal capillary we designed.
Subsequently, local currents were detected at the surface of the sample by using the scanning vibrating electrode technique.
Energy requirements were determined for the entire sample by using the Harris-Benedict equation; energy requirements for a subset of 25 patients were also determined by using indirect calorimetry.
Thereafter, the reaction was carried out with the spent sample by using the same reaction conditions mentioned in "Experimental section".
At the beginning of each of these periods the and each node of the BAN,, synchronize and exchange a new time sample,, by using the SPS-SE protocol.
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These sites were sampled by using the same methods, thus allowing us to describe changes in An. hyrcanus and Cx.
DNA was extracted from HBsAg positive samples by using the QIAGEN DNA Blood Mini kit (QIAGEN, Venlo, the Netherlands) according to the manufacturer's protocol.
DNA was extracted from DBS and fresh blood samples by using the NucliSense easyMAG automated extraction platform (bioMérieux, Boxtel, the Netherlands) according to the manufacturer's instructions.
For the Rt qPCR, cDNA was obtained from RNA samples by using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with oligo dT primers.
Coarse woody debris was sampled by using the strip-plot method to determine log density, mean log size, volume, and cover.
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