We prepared the PDMS samples, by mixing the silicone elastomer base and cross-linker (i.e., hardener) in the ratio of 10 1 by weight.
[H]-Dopamine was measured in the samples by mixing the homogenate 1 : 3 with Ultima Gold XR scintillation cocktail and counted using scintillation spectroscopy (Beckman, High Wycombe, UK).
With these relative abundance vectors, we generated 90 metagenomic samples by mixing the 5 bacterial genomes at the abundance levels defined in the vectors and sampling short reads from the mixture genomes with MetaSim [ 47].
In order to evaluate the ability of a specific platform to detect subtle changes in gene signals and differentials, we generated 3 new samples from the original AGO and BMO RNA samples by mixing the original pure BMO into AGO at a ratio of 1 4, 1 16, and 1 64, and the 3 new RNA samples were labeled as AG1BM4, AG1BM16, and AG1BM64, respectively.
To analyze the origin of chimeric RNAs and to discern if they are artifacts derived of library preparation or natural chimeric RNAs, we prepared different samples by mixing RNA of M. pneumoniae and P. aeruginosa at different ratios (1 1; 1:5 and 1 50), as well as non-mixed samples.
This study used DRS to measure reflectance patterns of 68 samples made by mixing samples from two soils with different clay content, three levels of organic carbon, three petroleum types and three or more levels of contamination per type.
For analysis, a reference sample made by mixing all the different samples was injected and a reference library was created by software assisted peak finding.
For instance, a sample prepared by mixing 80% sand and 20% silt was addressed by 20% silt content sample.
Specific potential lung toxicity of organic extracts of PM0.5 will be assessed in vitro on a total of 10 unique samples, obtained by mixing the organic extracts from all the samples individually collected from each town and each season.
Urine samples prepared by mixing 20 μL urine with 180 μL 50% aqueous acetonitrile containing 5 μM chlorpropamide.
