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Isolation of virus was conducted from PCR-positive sample by injecting mosquito homogenate supernatants into monolayers of BHK-21 and C6/36 cells.
To prevent carry-over between samples, the column was washed after each sample by injecting 1% Zwittergent (100 μL) on to the column and applying the following wash gradient: 100-20% B, 8 minutes.
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Mobility reduction factor is measured through differential pressure across the sandstone core samples by injecting CO2 before and after foam surfactant formulations.
Samples were measured by injecting 1 µL of sample and helium (2 mL min−1) was used as a carrier gas during which the oven temperature was increased from 80 to 290 °C at a rate of 4 °C/min and finally 30 min at 290 °C.
Samples were analyzed by injecting 18 µL of sample over a 0.1032 mm Prontosil 300-3-C18H (Bischoff, Germany) pre-concentration column (prepared in house) at a maximum pressure of 270 bar.
Polar samples were analyzed by injecting 1 μl with a split injection ratio of 5 1; lipophilic samples of 1 μl were analyzed using a 1 1 split injection ratio.
At the time of analysis, fecal samples will be thawed and a carboxen/polydimethyl-siloxane solid phase micro-extraction (SPME) fiber will be placed into the sample collection vial by injecting it through the septum in the cap.
At the time of harvest, mice were euthanized with carbon dioxide, after which BALF samples were collected by injecting 2×300 μl of sterile PBS to the trachea.
Samples were collected by injecting 0.2 mL of sterile saline into the dermis of the internal border of skin lesions with sterile insulin syringes.
Automated post-run internal mass scale calibration of individual samples was performed by injecting the calibrant at the beginning and end of each run via a six-port divert valve equipped with a 20-μL loop.
Sample carryover was evaluated by injecting a series of blanks (n = 4) after a high concentration standard (2000 ng L−1) in every sequence.
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