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It was further heated at 750°C for 4 h to find the percentage of carbon in the sample by calculating the reduction in weight.
Parceled indicators were computed for the present sample by calculating the mean response for the shortened list of items representing a particular subscale.
We examined the characteristics of the study sample by calculating the mean values of continuous variables and frequencies of categorical variables.
We determined the crossing points of the amplification curves for the variable and the common DNA tag region for each sample by calculating the second derivative maximum (LCS480 1.5.0.39 software).
TROP2 overexpression was evaluated for each tissue sample by calculating a total immunostaining score as the product of a proportion and intensity score.
We then created a normalization factor for each biological sample by calculating the mean of that sample's within-group transcript fold change across all transcripts.
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The feature data of each class are obtained from the samples by calculating the average feature data of all samples.
Natural moisture content was determined in both soil samples by calculating the ratio of the weight of water to the weight of solids in 500 g of each sample according to ASTM D2216-90.
For the tree based on mRNA expression, we created pooled samples by calculating the mean expression value for each gene based on expression levels in two individuals of the same species (Table S2).
A script automatically visualizes the results as cluster diagrams for each SNP and genotypes the samples by calculating allelic fractions (AFs).
We estimated population structure between all sites sampled by calculating ϕST, taking into account haplotype frequencies and the genetic distance between haplotypes, in Arlequin 3.0 [ 42].
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