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Sample buffer was added to 1× final concentration.
One part of the sample was diluted with one part of the Laemmli sample buffer.
The protein complexes were then washed with PBS and eluted in SDS PAGE sample buffer.
The concentrated purified cytosolic protein was mixed with 4x Laemmli sample buffer.
The cleavage reactions were stopped by adding SDS sample buffer and heating to 90 °C.
Cell lysates were denatured in reducing Laemmli sample buffer for 5 min.
The pellets and chromatin were resuspended in 2× SDS sample buffer.
The beads were eluted with SDS sample buffer and subjected to SDS-PAGE.
Beads were then extensively washed and boiled in sample buffer containing 1% SDS.
Reactions were stopped by addition of reducing SDS sample buffer and boiling for 5 min.
The stimulated cells were rinsed with ice-cold PBS and lysed in SDS-PAGE sample buffer.
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