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Serum AST and ALT levels were assessed by using commercial kits (Roche Diagnostics, GmbH, D-68298, Mannheim, Germany) in Roche-Hitachi Modular Autoanalyzer (Roche Diagnostics, GmbH, D-68298, Mannheim, Germany).Bayer Opera autoanalyzer was used to measure bilirubin levels by wavelength, 550 nm; temperature, 37°C; and instrument set for endpoint with sample blanking mode.
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A typical analysis sequence consists of blank, standard, blank, sample 1, blank, standard, blank, sample 2, and so on.
10 sample blanks were also prepared following the same procedure.
A sample blank digestion was also prepared after every 10 samples.
The sample blank was prepared by using methanol instead of ABTS solution.
The sample blank was prepared by using methanol instead of FeCl3 solution.
Sample blanks were prepared in a similar manner, except DNPH was excluded.
The sample blank was prepared by using ethanol or methanol instead of DPPH solution.
Sample blanks consisting of deionized water were collected in the field and analyzed, yielding method blank sample concentrations that were consistently below analytical detection limits.
The determination of all enzyme activities was carried out in triplicate, and sample blanks were taken for each sample.
Sample blank was prepared in the same manner except that methanol was used instead of ABTS solution.
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