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The last SF sample available from each knee (post-treatment) underwent synovial effusion analysis.
As is typical with longitudinal studies, there was significant reduction in the sample available from the original cohort over time.
Inclusion criteria were severe sepsis, pre-intervention whole blood TEG measurement and a plasma/serum research sample available from baseline (pre-intervention) for analysis of endothelial-derived biomarkers.
Validity was assessed by comparing average allele frequency estimates across 5 DNA pools to those from the independent sample available from HapMap [ 11] and NetAffx™ [ 48].
Due to the limited amount of sample available from each infant, preliminary work was necessary to determine which assays and conditions give the most useful information.
Thus, any potential predictive effect of the LCS6 variant allele is likely to be too small to be demonstrated with the patient sample available from the NORDIC-VII study.
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Patients with severe sepsis or septic shock diagnosed on day one and who had plasma samples available from admission to the ICU were included in the current study.
Serum samples available from 4 critically ill patients exhibited HI titres of 40 320.
Seven paired samples available from the right ventricles of patients were processed in the same way.
The Dai and Cambodian samples were included since there were limited numbers of samples available from the Vietnamese group.
Therefore we analyzed an additional SEPT9 replicate using 10 fold-diluted samples available from the original total bis-DNA measurements.
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