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The resulted unbalance and incompleteness nature of sample availability in genomics study, without appropriate attention in sample-to-batch allocation, could lead to drastic batch effects.
However, it is not always possible or appropriate to undertake such a full evaluation in wildlife studies due to time and budget constraints as well as sample availability, in particular accessing known positive and negative controls.
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Post-digest labeling with iTRAQ or ICAT isotopic labels [32] is also difficult due to limited sample availability, limitations in instrumentation (one-third rule with the LTQ spectrometer restricting detection of the labels in the low molecular weight range) [33], and the labile nature of phosphates and labels which causes reduced fragmentation and detection in MS/MS spectra [34].
The high cost of microarray experiments and limited sample availability resulted in a small sample size for each treatment group.
Unfortunately, RBP4 plasma levels in the Rotterdam Study are not available because of limitations in sample availability and lack of a readily available and reliable method to measure these levels in large populations and people with insulin resistance [ 34].
However, due to limitations in sample availability (precluding the study datasets with counts in the hundreds-to-thousands of samples), a two-way approach to pathway analysis was followed based on mining both large scale databases and highly curated knowledge bases –with all interactions taken from actual experimental data – implemented within the causal network philosophy [ 29, 30].
Up to now, in part due to the limitation in sample availability, few studies on normal human tissues have been reported.
It is also worth noting that there only ∼31 60% of genes from this 187 gene signature that were used in clustering analyses in each of validation datasets due to the specificity of platforms, sample availability and missing values in microarray experiments.
Selection of patient samples was based on sample availability and drugs were run in parallel.
These species were chosen based on sample availability following elemental analysis, and in order to provide a broad taxonomic range (Table 1).
39 41 However, in our sample, availability of social support and isolation both had stronger relationships with resilience than perceived adequacy of support.
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CEO of Professional Science Editing for Scientists @ prosciediting.com