Sentence examples for sample as starting from inspiring English sources

Microarray analyses were performed using 3 µg of total RNA of each sample as starting material and 10 µg cRNA per hybridization (GeneChip Fluidics Station 400; Affymetrix, Santa Clara, CA).

Thereafter, the Illumina Truseq RNA Sample Preparation kit v1 protocol was followed directly, using 4 μg of RNA per sample as starting material.

Using a total of 240 μL of the composite sample as starting material, we acquired a total of 62,686 MS/MS spectra from 12 different LC-MALDI-TOF/TOF runs.

This is because the design of the 2008 EDAD used a representative Spanish sample as starting point but only obtained more detailed information about lived health from the subpopulation with limitations in biological health.

After isolation of RNA, as described in the section 'RNA isolation and quantitative PCR', the Illumina Truseq RNA Sample Preparation kit v2 protocol (Illumina, San Diego, CA) was followed using 500 ng of RNA per sample as starting material.

Also, in Germany, and using a general population representative sample as starting point, Mueller et al. [ 10] confirmed that about 7% of the people in this country were compulsive buyers.

The method consists of two steps: first, we generate asymptotically uniform random samples in a convex domain via Hit-and-Run method and then apply local descent procedure based on conditional gradient for a concave function treating samples as starting points.

Aerodynamic levitation experiments required solid, nearly spherical samples as starting material.

We also carried out grinding experiments on borehole core samples and sericite standard samples as starting materials and investigated the effect of mechanical comminution on the IC and illite peak intensity of the experimental products.

Each microarray experiment was performed four times using, in all cases, independently isolated RNA samples as starting material (four biological replicates).

In order to validate the list of differentially expressed/regulated genes revealed by microarray analysis, we randomly selected a subset of 18 genes for analysis by real-time quantitative PCR (RT-qPCR) using the original RNA samples as starting material.