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Bacterial RNA was isolated from each sample as previously described [13].
DNA was isolated from patient blood sample as previously described [33].
Yields were converted to femtograms and normalized to the 18S mRNA level per sample, as previously described [51].
Before performing the AQAMA assay of LINE-1 methylation status, SBM was applied on each DNA sample as previously described [32].
DNA methylation was monitored by qPCR analysis of mock-treated and McrBC-digested portions of each sample, as previously described [27].
The LTL163a tumor line (www.livingtumorlab.com) is derived from a high-grade prostate cancer sample obtained from a patient, and the xenograft tumor transplant line was developed from this clinical tissue sample as previously described [33], [34].
Similar(8)
Sample preparation was as previously described.
Blood samples were collected as previously described.
CVM samples were collected as previously described.
Organic acids, including citrate, were extracted from leaf samples as previously described (Phillips and Jennings 1976), with some modifications.
Bisulfite sequencing was performed in 9 samples as previously described [20].
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