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Separate hybridization cocktails were made using 15 micrograms of fragmented cRNA from each sample as per Affymetrix's protocol.
The heated buffer and LNAdt blocker (Genesphere) was then added to the collected sample, as per manufacturer's instructions.
First strand cDNA synthesis was carried out using Superscript III reverse transcriptase (Invitrogen) and 3 μg random hexamer primers (Illumina) per sample as per the manufactures' instructions.
The target and control reactions were run in separate tubes on the same plate for each sample as per the manufacturer's protocol.
Three parts (1.5 ml) of the sample reagent were added to one part (0.5 ml) of the sediment sample as per the manufacturer's guide.
First strand cDNA synthesis was carried out using Superscript III reverse transcriptase (Invitrogen) and 3 ug random hexamer primers (Illumina) per sample as per the manufactures' instructions.
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In early 2013, Tesco, the British supermarket chain, made a startling revelation: some of its frozen beef patties contained horse meat, one sample as much as twenty-nine per cent.
To compare global miRNA expression levels between these samples we normalized all mapped miRNA transcripts in each sample as reads per million.
The target cDNA generated from each sample was processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System.
Hybridization: the target cRNA generated from each sample was processed as per manufacturer's recommendation using a GeneChip Instrument System (Affymetrix).
1 mL CSF and 2 5 mL of clotted blood sample were collected as per standard procedures.
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