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Calculate the analytes -catechin and -epicatechin in the original sample as follows: Analyte in sample [µg/g] = assay concentration of analyte [µg/mL] * × [mL]/W sample [g].
C/N ratio was obtained by taking the ratio of Spercentt carbon to nitrogen in each sample as follows: C/N = frac{SOC ;}{TN ;}where C/N the ratio of carbon to nitrogen, SOC the concentration to carbon in the soil sample, TN the concentration of total nitrogen in the soil sample.
The CD content was calculated from the absorbance and the final concentration of the sample as follows: {text{CD }} = {text{ A}}/{text{C}} times {text{P}},where A is the absorbance of the sample at 233 nm; C is the final concentration of the sample after dilution (grams per 100 mL of isooctane); and P is the path length of the cell (cm).
PD was computed for each sample as follows: if measurements from both eyes were recorded as "good" the two pupil diameters were averaged.
A lytic enzyme cocktail was prepared at the time of extraction and added to each sample as follows: 50 µl Lysozyme (450 kU ml-1), 6 µl Mutanolysin (25 kU ml−1), 3 µl Lysostaphin (4 kU ml−1) and 41 µl TE50 for a final volume of 100 ml per sample.
A lytic enzyme cocktail was prepared at the time of extraction and added to each sample as follows: 50 µl Lysozyme (450 kU ml−1), 6 µl Mutanolysin (25 kU ml−1), 3 µl Lysostaphin (4 kU ml−1) and 41 µl TE50 for a final volume of 100 µl per sample.
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Victims were sampled as follows.
Thereafter, the root system was excavated and sampled as follows.
The procedure that we use for selecting the samples as follows: 1.
The myo-inositol content decreased from the oldest samples to the younger samples as follows: first style, about 5%; second style, about 1%; third style, about 0.3%; and fourth style, <0.1% (Additional file 1: Figure S3).
Statistically significant windows were determined by comparing actual moving average values to a null distribution obtained by permutation sampling as follows.
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