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In addition, ΔCtendo was calculated by using endogenous control Ct values for the same calibrator sample as above, and subtracting subsequent endo Ct values from that calibrator [55].
For histology and fluorescence imaging analysis, 12-µm-thick sections were prepared from the same OCT embedded frozen sample as above.
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Then, randomly generated observations from that normal distribution were sampled as above.
The DUX4 5' BSS analysis was tested on the same eight genomic DNA samples as above.
From those who agreed, the same data and samples as above were obtained.
The same samples as above were sequenced using commercial kits and protocols provided by Life Technologies, Carlsbad, USA.
In subsequent trials, fish were sampled as above, but at seven and 14 dpe (Trial 2) and at 28 and 43 dpe (Trial 3).
We clustered the samples as above and compared these clusters to the class labels predicted by the decision tree model with Cohen's kappa statistic [ 24, 25].
Specifically, we evaluate the difference between the two methods' aROC on each bootstrapped sample generated as above.
Peaks of two samples identified as above were merged as candidate differentially methylated regions (DMR).
Bovine bladder samples collected as above described were stored at −80°C until further processed.
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