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Evaluation of whole genome amplified DNA (wgaDNA) for genotyping purposes has shown that the allele fractions in the original sample are retained provided that a sufficient amount of genomic DNA is used [ 19, 20].
Thus, only the insertions that are unique to the TD sample, have sufficient coverage and close to no discordant reads at the corresponding locus in the ND sample are retained as somatic.
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The injected sample is retained along the entire LC LCA column.
Characterization by transmission electron microscopy (TEM) revealed that the microstructure of the shock-consolidated sample was retained at the nanoscale.
If the average distance of a test sample was lesser than or equal to the threshold value, the test sample was retained within the AD.
As the entire system is self-contained and the sample is retained in the corer, it was found ideal for extracting very soft sediments which could not be captured by existing corers.
Cells in the residual volume of the initial sample were retained and used for controls.
The other half of the sample was retained as an unexposed control.
Twenty percent of each sample was retained as a 'Total' fraction, and precipitated in 4 volumes of methanol.
A 5 µL aliquot of each sample was retained to measure total bisulfite treated DNA using the real-time PCR assay (HB14) described in Supplementary Table S1.
A 5 µL aliquot of each sample was retained to measure total genomic DNA using the real-time PCR assay (CFF1) described in Supplementary Table S1.
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