In the AFLP phylogenies, the D. capensis samples are nested within the D. delphis clade.
Similarly, the log-transformed spectral index was modeled as time of collection (daytime or nighttime), and the biological samples were nested within time of collection under the assumption of normality.
The AFLP data support this close relationship, in fact, with the S. longirostris samples being nested within the S. clymene clade, suggesting a recent common ancestry for these two taxa.
The spectral count data on each peptide identified at both time points was analyzed using a Poisson model where the spectral count was modeled with collection time (daytime or nighttime) and the biological samples were nested within the time of collection.
Therefore the statistical approach was chosen to take into consideration the multilevel structure of data from our sample where the pen faecal samples (level 1) are nested in herds (level 2).
Due to the sampling design, Sites are nested within Regions, denoted as Site(R), and M. capitata colonies are nested within Sites, denoted as Colony(S R)).
Thus, the study has a nested sampling design: individuals are nested in healthcare practices, both are nested in geographic areas, which are nested in countries.
20 The HBSC employs a multistage cluster sampling strategy in which the sampling units are classes nested within schools, which in turn are nested in their school boards, then provinces/territories.
Sampling year was nested in site in order to test for local year-to-year variation in infection presence.
A pre-post intervention two-sample design is nested within the CIS arm to assess the incremental effectiveness of the CIS plus financial incentives (CIS + FI) compared to the CIS without FI on study outcomes.
