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X-rays passing through the sample are converted to visible light at a scintillator, and the resulting light passes through a traditional microscope optics chain and is recorded using a high-resolution CCD (Cooke PCO4000).
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This analog signal is periodically sampled, with each sample being converted into a digital equivalent by means of an analog-to-digital (A/D) converter.
Total RNA (200 ng) from each sample was converted to single-stranded cDNA using the Quantiect RT-PCR (Qiagen, Germantown, MD) with random primers 10 µM.
Total RNA (1000 ng) from each sample was converted to single-stranded cDNA using the Quantiect RT-PCR kit (Qiagen, Germantown, MD) with 10 µM of random primers.
RNA concentration and purity were quantified using a NanoDrop 2000c (all samples had a 260 nm/280 nm absorbance ratio of 2 to 2.1). 1 ug of RNA per sample was converted to cDNA using the AffinityScript QPCR cDNA synthesis kit (Agilent Technologies).
The volume of NaOH required to neutralize the sample was converted to total surface charges.
The total amount of platinum measured from the sample was converted into the amount of cisplatin.
Each RNA sample was converted to cDNA by using iScript™ cDNA synthesis kit with reverse transcriptase (Bio-Rad, Hercules, CA, USA).
For the stomach content data, the mass of prey groups present within each sample was converted to percentage composition.
The RNA concentration in the samples was determined spectrophotometrically and 1 µg of RNA per sample was converted to cDNA using Superscript II reverse transcriptase from Invitrogen.
1.5 µg of total RNA per sample was converted into labeled cDNA using the GeneChip® WT Sense Target Labeling kit (Affymetrix).
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