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Oligoclonal IgG analysis was performed using a commercial kit on Hydrasys instrument (Sebia, Évry Cedex, France, Cat. No. 4355) according to the manufacturer's instructions (except that the amount of the sample applied was 15 μl instead of 10 μl recommended in the instructions).
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The properties of the samples applied are given in Table 2.
To estimate sample size, proportional sampling was applied and sample size of at least 320 persons was calculated.
The standardized biological sample collections applied have been described in detail previously [5], [6], [7], [48].
After the samples were applied, the cartridges were washed with 2 mL of 2.5 mM phosphoric acid (pH 3.8).
To obtain a representative sample, stratified random sampling was applied to select study participants from the source population.
Therefore, a non-random sampling method, network sampling, was applied, which was based on referrals from early members (key persons) already in the sample.
The adhesive was applied by a robot and the second "sample part" was applied manually.
To control the precision of sampling, stratified cluster sampling was applied.
The random sample process was applied to each hospital.
Appropriate sample weight was applied to conduct the analysis.
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