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The loss can be partly ascribed to the volume of sample applied and eluted from the stabilization product.
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To estimate sample size, proportional sampling was applied and sample size of at least 320 persons was calculated.
Then 350 μl 70% ethanol was added, mixed well by pipetting and the sample applied to a MinElute column (RNeasy Micro Kit), and centrifuged at 8000 × g for 30 seconds.
The HiTrap Protein G HP column (GE Healthcare) was equilibrated with binding buffer and the sample applied.
Imidazole was added to 40 mM and the sample applied to a Ni2+-immobilized column.
In conducting the research, a purposive sampling were applied and a sample of 100 recipients of AIM microcredit program loan were selected via their weekly meeting.
First, we wanted to ensure that the sample could be reliably cut with a large sample bias applied and the diamond knife sitting at ground.
Then, 1 μl of trypsin-digested sample was applied and dried.
A Gibbs sampler was applied and a total of 40000 iterations of sampling was performed, with a burn-in of 10000 iterations.
Potentiometric sensing utilizing textile-based micro-volume sampling was applied and evaluated for the determination of clinically (Na+, K+, Cl–) and environmentally (Cd2+, Pb2+ and pH) relevant analytes.
Then samples were applied and chromatography performed in the same mixture of solvents [83].
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