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Live capture efforts often involved trapping and euthanizing live animals, whereas carcass-sampling efforts involved sampling animals that were found dead.
For sampling, animals that have shown torpor at least three times were designated depending on their time of inactivity after an arousal episode as early torpor (TE; 8 h of inactivity; n = 5 for Western blot and n = 2 for immunohistochemistry) or late torpor (TL; 36 48 h of inactivity; n = 5 for Western blot and n = 3 for immunohistochemistry).
One boar with a clinical score of 4 was culled before day 0. Mange mites were isolated from 8 out of 95 sampled animals that remained in the study.
Among 289 alpine ibex observed in the massif, 24 were killed (22 randomly sampled animals that showed 2 diagnostic lesions at necropsy [arthritis in the knee and mammary abscesses]) and 2 diseased animals (arthritis in the knee and orchitis), and samples from these animals were subjected to serologic analysis.
Each reported replicate represents a pooled sample of animals that were selected from different crosses within a single experiment.
To increase sample size, animals that did not eat any of the offered crickets were later assigned to the control group (N = 6).
DNA was isolated from skin biopsy samples of animals that carry a single copy of the modified allele and with or without tamoxifen-inducible global Cre transgene.
Extracts of plasma and liver samples from animals that had received DMU 212 exhibited several peaks in addition to that of the parent drug.
Figure 7 shows the results of the renal cell necrosis assay in samples from animals that had been treated with either gentamicin (Fig. 7J) or Cd2+ (Fig. 7L).
As shown in Table 3, samples from animals that did not present emaciation or parasitic infection figured among the least contaminated.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com