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Male NMRI mice were tested in the object location model which is based on the animals' inherent interest to examine changes in their environment: The animals' propensity to explore relocated objects in relation to unaltered objects, presented in two different sessions (sample and trial), was studied.
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Importantly, the goal remained in a fixed location for the sample and test trial, and during the test session, all the maze arms were open (see Figure 1).
The same start position was never used between the sample and test trial, to insure navigation based on memory of spatial position rather than turn angle.
Based on a random schedule, the position of the submerged platform was changed after each sample and test trial to eliminate the use of spatial cues.
Each session consisted of three paired trials, with intervals of 60, 120, or 180 seconds between the sample and test trials (the intervals do not include the 10 seconds required for repositioning the cylinders and platform).
As a control, all of the mice were tested in a static spatial separation trial during which there was no change in the object location between the sample and test trials.
The non-matching-to-sample task consisted of a series of paired sample and test trials.
All quadrants were used equally for locating cues in the sample and test trials and the platform was positioned randomly.
Rats received five daily sessions and each session consisted of four pairs of sample and test trials.
The time that the mouse spent exploring the objects was recorded during both the sample and test trials.
For each rat, the time spent exploring the objects during sample and test trials was scored offline from videotapes.
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