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A quenched amorphous sample and three specimens annealed above the glass transition for different times have been analysed.
Two biological replicates for each sample and three technical replicates for each biological replicate were used for RT-PCR analysis.
For statistical analysis, six different substrate fields were measured per sample, and three separate samples were analyzed.
The SEM images of six different substrate fields were measured per sample, and three separate samples were measured for each nanopore surface.
For community testing, total 60 views were obtained for each WWTP (ten views for each well, two wells for each sample and three samples for each WWTP).
Thirty oocytes composed one sample and three samples were assayed for each treatment.
Triplicate wells were performed for each sample and three replicate samples of flies were run for each treatment condition.
This procedure was carried out on ninety individual flies per sample, and three samples for each sex and each genotype was collected.
The amount of kinase reaction product used for SDS-PAGE was strictly controlled (20 µl) for each sample, and three samples were analyzed for each treatment.
A negative control without cDNA, technical replicates on three independent cDNA samples (derived from the same RNA sample), and three independent biological experiments were performed in all cases.
Three Stem Cell Array plates were run for each undifferentiated sample and three ECM plates were run for each NPC sample, for a total of 12 plates of each type.
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CEO of Professional Science Editing for Scientists @ prosciediting.com