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Using the SOLiD V3.0, 50 bp sequencing reads were generated for each sample and resulting high quality reads were compared/aligned to the existing genome sequences of the H. influenzae strains Rd KW20, 86-028NP and 10810 to determine sequence homology using the SETS software tool that is integrated into the SOLiD platform.
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Untreated seedlings were imaged before and after recording the auxin treated samples and resulting average was defined as T0.
Normal mucosas (100 200 mg) were handled like tumour samples and resulting DNAs were analysed for candidate-gene polymorphisms.
However, this was likely to due to the sample sizes and resulting low statistical power.
This approach involved the analysis of 40-ml field samples and resulted in a quantification limit based on both sample and lysis buffer volumes.
The final gene list was generated by selecting transcripts with 1.5-fold higher expression in the pregnant than non-pregnant blood samples and resulted in 247 gene transcripts.
He was also involved in study design, data collection, quantitative analysis of the samples and result interpretation.
The similarity of results between this older sample and results derived by others from younger participants is notable.
The assay was replicated thrice for each sample and result was taken as mean ± standard deviation.
Three separate aliquots were taken from each soil sample and results are shown.
One hundred glomeruli were counted per sample, and results are presented as percentage of glomerular crescents.
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CEO of Professional Science Editing for Scientists @ prosciediting.com