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We then added the DDM and CCNM estimates for the other three fossil hominins to the sample and ran another t-test.
We extracted a plasma sample and ran it in triplicate on the same day for intraassay precision.
All PCRs were set up using a Biomek 2000 robot (Beckman), in a 10 µl volume in 384-well plates with six replicates per sample and ran in an ABI 7900 Sequence Detection System (Applied Biosystems) with the following conditions: 50°C for 2 min, 95°C for 10 min and 50 cycles of 95°C 15 s/60°C for 1 min. In total, 11 520 qRT PCR reactions were performed.
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Expression of the target genes was measured relative to that of RpL32 (rp49), in order to normalize for sample and run to run variations.
MS did the collection and freezing of MRSA samples and ran PCR tests.
We isolated RNA from these samples and ran libraries on a U133 plus 2.0 Affymetrix array.
BH developed the heterotrimer assay, measured heterotrimer and BLyS levels in serum samples, and ran the heterotrimer signaling assays.
We boiled the beads in sample buffer and ran SDS-PAGE.
Extracts were boiled with sample buffer and ran on 8 or 12% SDS-PAGE gels.
Researchers from the University of Mississippi and the University of Southern Mississippi are aboard the boat taking samples and running instruments.
Manhattan College is providing students and the sophisticated equipment needed to draw storm water samples and running tests to determine what is in the samples.
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