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For every sample and quantitative target extracted intensities from corresponding integration bin were summed.
This was still the case when Europe-defined PN2 cases which are based on the criteria "non-protective sample and quantitative culture" were considered as clinically defined PN.
Total RNA was extracted from zebrafish embryos (∼30 embryos per sample) and quantitative PCR carried out as described in ref. (18) on an ABI 7000 sequence detection system (Applied Biosciences), where possible primers were designed to span an intron.
In this technique (reviewed in [ 12]) RNA expression levels are measured by direct sequencing of RNA in the sample, and quantitative expression levels are derived from number of reads mapping to each locus.
From a previous research sample and quantitative study of 398 GPs representative of Quebec's GP population [ 19, 27, 28], 60 GPs were selected for further investigation, i.e. for a more in-depth examination of GP management of mental disorders in view of the results of the first study.
A caveat of our culture approach is that we cannot readily quantify the amount of fungi in a sputum sample and quantitative PCR (QPCR) may be an advantage in this respect; however, QPCR is only able to detect the fungus to which specific primers have been designed.
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An ArF excimer laser system (λ = 193 nm) with imaging optics for controlled UV ablation and simultaneous petrographic viewing was designed specifically for representative sampling and quantitative multi-element analysis of microscopic fluid, melt and mineral inclusions beneath the sample surface.
Table 1 The preparation conditions for the samples and quantitative ratio between oxygen and cobalt Deposit V e (kV) I e (nA) O/Co I(L2)/I(L3) 1 30 0.044 0.85 0.27 2 30 2.400 0.04 0.30 Summary of growth parameters, beam energy (V e) and current (I e), EELS quantification ratio between oxygen and cobalt and the average L2/L3 intensity ratios in Co L2,3 edge (see text for details).
To facilitate individual sampling and quantitative collection of all voided faeces without handling the animal, the method described by Touma and colleagues was used [31], [32].
Decoding of samples and quantitative mapping to the input amplicon sequences concludes the experimental workflow.
Image acquisition, dendrite sampling and quantitative analyses were performed as previously described (Nwabuisi-Heath et al., 2012).
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